Reversed passive latex agglutination assay for detection of toxigenic Corynebacterium diphtheriae
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منابع مشابه
Reversed passive latex agglutination assay for detection of toxigenic Corynebacterium diphtheriae.
A reversed passive latex agglutination (RPLA) assay for determining the toxigenicity of Corynebacterium diphtheriae is presented. Rabbit antitoxin antiserum was raised by using commercially available diphtheria toxoid. This antiserum reacted with the diphtheria toxin when the culture supernatant was assayed by Western blotting, and it did not cross-react with other extracellular antigens. Affin...
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Introduction A series of test kits for detecting bacterial toxins have recently been put on the market byOXOId Limited. These kits employ reversed passIve tatex agglutination (RPLA) as the assay method and, in this article. the development, characteristics and value of the method for detecting bacterial toxins are briefly reviewed . Bacteria produce enzymes and toxins With vanous biological act...
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The systemic symptoms of diphtheria are caused by the tox-encoded diphtheria toxin (DT) which is produced by toxigenic Corynebacterium spp. Besides the classical agent C. diphtheriae, the zoonotic pathogen C. ulcerans has increasingly been reported as an emerging pathogen for diphtheria. The reliable detection of toxigenic Corynebacterium spp. is of substantial importance for both diphtheria su...
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متن کاملExfoliative toxin detection using reversed passive latex agglutination: clinical and epidemiologic applications.
A rapid and simple method for detecting exfoliative toxin serotypes A and B from clinical isolates has been developed as a test kit (EXT-RPLA; Denka Seiken Co. Ltd., Niigata, Japan). This method is based on reversed passive latex agglutination. The detection limit of the EXT-RPLA observed for purified exfoliative toxin serotypes A and B was 1 ng/ml. We evaluated the clinical and epidemiologic u...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1997
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.35.12.3147-3149.1997